Medical genetics, Cytogenetics Techniques and diagnostic methodology

Module Diagnostic methodology of Clinical Pathology

The  objective of the Course is to enable the student to acquire the basic notions for the critical evaluation and interpretation of the results of the main laboratory diagnostic techniques.

Frontal classes

Mandatory at 70%

Polyclonal and monoclonal antibodies. Production techniques: Hybridomas and Phage Display

ELISA: direct, indirect, sandwich and competitive ELISAs; Standard curve and interpolation of unknown values

Flow cytometric techniques: basics; compensation; cell cycle study; analysis of apoptosis

Electrophoresis (agarose gel electrophoresis, PAGE electrophoresis, protein electrophoresis in native and denaturing conditions, capillary electrophoresis, isoelectric focusing), Western Blot 

First and Next Generation Sequencing; Sanger sequencing, Maxam-Gilbert sequencing, pyrosequencing, Illumina sequencing, nanopore sequencing

Examples of clinical applications of laboratory techniques: serum proteins electrophoresis; autoantibodies

Abbas, Lichtman, Pillai. Immunologia cellulare e molecolare. Edra; 9° edizione 2018

Mauro Maccarrone. Metodologie Biochimiche e Biomolecolari. Zanichelli. 2019

Oral examination on the topics discussed in the classes