Diagnostic methodology of Pathological AnatomyModule diagnostic Cyto-pathology
Academic Year 2022/2023 - Teacher: Lidia PUZZOExpected Learning Outcomes
At the end of the course the student will have to know the main types of diagnostic cytopathology exams, be able to set up and color the cytological preparations, know the basic principles of reactive and neoplastic cellular pathology; it must also be able to apply the general concepts of cytopathology to the main types of cytological samples.
Course Structure
Biweekly.
Should teaching be carried out in mixed mode or remotely, it may be necessary to introduce changes with respect to previous statements, in line with the programme planned and outlined in the syllabus.
Detailed Course Content
Diagnostic cytopathology: advantages and limitations
The different cytologic samples: needle and aspiration cytology
Technique of cytological preparations: smear, thin, cell-block, cytocentrifugation
Fixation and histochemical staining of cytological samples
Techniques of immunocytochemistry, flow cytometry and FISH in diagnostic cytology
Evaluation of cytological samples: criteria of adequacy
Evaluation of morphological characteristics of the cells: normal cells, hyperplastic, atypical cells
False negatives and false positives
Diagnostic Cytopathology of thyroid
Diagnostic Cytopathology of the lung
Diagnostic cytopathology of the female genital tract: PAP tests and breast cytology
Diagnostic Cytopathology of the urinary tract
Diagnostic Cytopathology of the effusions
Textbook Information
Anatomia Patologica- Le basi- Luigi Ruco, Aldo Scarpa. Edizioni UTET
Course Planning
| Subjects | Text References | |
|---|---|---|
| 1 | Indicazioni all'esame citologico: vantaggi e limiti I diversi tipi di esame citologico: citologia esfoliativa ed agoaspirativa Allestimento dei preparati citologici: striscio, strato sottile, cell-block, citocentrifugazione | Anatomia Patologica- Le basi- Luigi Ruco, Aldo Scarpa. Edizioni UTET |
| 2 | Fissazione e colorazioni istochimiche dei preparati citologici Applicazione di tecniche speciali di immunocitochimica, citofluorimetria e FISH nella diagnostica citologica | Anatomia Patologica- Le basi- Luigi Ruco, Aldo Scarpa. Edizioni UTET |
| 3 | Valutazione dei preparati citologici: criteri di adeguatezza del prelievo Valutazione delle caratteristiche morfologiche delle cellule: cellule normali, cellule iperplastiche, cellule atipiche Falsi negativi e falsi positivi | Anatomia Patologica- Le basi- Luigi Ruco, Aldo Scarpa. Edizioni UTET |
| 4 | Citopatologia diagnostica della tiroide Citopatologia diagnostica del polmone | Anatomia Patologica- Le basi- Luigi Ruco, Aldo Scarpa. Edizioni UTET |
| 5 | Citopatologia diagnostica dell'apparato genitale femminile: PAP test e citologia mammaria | Anatomia Patologica- Le basi- Luigi Ruco, Aldo Scarpa. Edizioni UTET |