Diagnostic methodology of Clinical microbiology and ParasitologyModule Microbiology Diagnostic Techniques
Academic Year 2022/2023 - Teacher: Mario SALMERIExpected Learning Outcomes
TECHNIQUES FOR DIAGNOSTIC MICROBIOLOGY
Knowledge of applications foundation for microbiological sciences in the various sectors of health interest
Knowledge of the main microbial identification techniques and acquisition of the skills to apply the same in health care
Knowledge of the "Guidelines for sterilization on the activity as a collective protection against biological agents for the operator in health facilities (Legislative Decree no. 81/2008 and subsequent amendments)"
Course Structure
TECHNIQUES FOR DIAGNOSTIC MICROBIOLOGY
Lectures
Should teaching be carried out in mixed mode or remotely, it may be necessary to introduce changes with respect to previous statements, in line with the programme planned and outlined in the syllabus.
Learning assessment may also be carried out on line, should the conditions require it.”
Detailed Course Content
TECHNIQUES FOR DIAGNOSTIC MICROBIOLOGY Principles of Microbiology
Microbes and metazoa
Principles of microbial nutrition and metabolism
Methods of microbial taxonomy and nomenclatureMetodi biochimici
Molecular methods
Other methods (not molecular) New approaches to taxonomy Omic Sciences
Diagnostics of bacterial diseases
The methods of sampling
Transport media for bacteriology
Microscopic examination Staining techniques
Direct diagnostics Cultivation methods
Culture media
Aerobic and anaerobic growth
Microorganisms that require special nutrients and/or cells for growth Biochemical tests for identification
Manual methods
Automated methods Blood Culture
Culture independent and molecular techniques Immunofluorescence
Enzyme immuno assay Other methods Molecular methods
Hybridization Probes
Amplification techniques
Typing systems
Other culture independent techniques
Indirect diagnostic: Serological methods
Immunofluorescence Enzyme immuno assay Other methods
Susceptibility testing and bioassay
In vitro antibiotic susceptibility testing
Dilution methods Diffusion methods Automated methods
Bioassays
Pyrogenes and bacterial endotoxins Pyrogenes assay
Assay for bacterial endotoxins (LAL test) Biological Assay of antibiotics
Assay of interferons
Assessment of the activity of disinfectants
Diagnostic of viral diseases
The methods of sampling Cultivation method
The components required for cell culture media. Primary and Secondary cell cultures Semi-continuous diploid cells (Cell strains) Continuous cell lines
Culture independent and molecular techniques Enzyme Immuno assay
Immunofluorescence Molecular methods
Hybridization
Probes
Amplification techniques Typing systems
Other culture independent techniques Serological methods
Immunofluorescence Enzyme immuno assay Other methods
Antiviral chemotherapy
The assay of substances with potential antiviral activity
Diagnostic of fungal diseases
The methods of sampling Microscopy and culture
Staining techniques
Mycological culture media
Biochimical test for fungal identification
Manual
Automated
Culture independent and molecular techniques
Immunofluorescence Enzyme immuno assay
Other methods
Molecular methods Hybridization
Probes
Amplification techniques
Typing systems
Other culture independent techniques
Serological methods Immunoflurescence
Enzyme iImmuno assay
Other technique Antimycotic susceptibility besting
Dilution methods Yeast Molds
Other technique
The microbiological quality control and hygiene environment work [Notes on guidelines for the activity of sterilization as a collective protection against biological agents for the operator in health facilities (D.Lgs. 81/2008 e s.m.i.)]
The sterilization process and its enterprise management People involved
Structural and technological requirements.
Large / medium-sized hospitals
Small hospitals Operating protocols
Harvesting
Decontamination
Washing (manual, ultrasaund, automated)
Personal protective equipment [gloves, protective clothing, respiratory protection devices, for face protection (face shield or equivalent)]
Rinsing, drying, inspection and maintenance
Medical paper, envelopes and rolls in paper-laminated polymeric film, a polymeric material composition of various types usable in sheets, polyolefin and similar materials suitable for use in rolls or tubular, reusable Material: container
Sterilization (steam sterilization, with ethylene oxide, with other physical methods, gas plasma of hydrogen peroxide, peracetic acid solutions
Controls on environments and the process steps
Food Microbiology - HACCP System
HACCP system
Description of the HACCP system
Preliminary phases of the application system Main features of HACCP system
Principles 1 to 7 Food microbiology
Meaning of microorganisms in food
Unfermented foods
Meat, poultry, eggs, seafood, vegetables and fruit Fermented foods
Fermented Vegetables, bread, cheese, fermented milk, fermented meat products
Textbook Information
TECHNIQUES FOR DIAGNOSTIC MICROBIOLOGY
Koneman's - Color Atlas and textbook of Diagnostic Microbiology 7ed. Wolters Kluver - International Edition ISBN-13: 978-1-4511-8935-3
Other informations and papers will be available in Studium
Course Planning
| Subjects | Text References | |
|---|---|---|
| 1 | TECNICHE IN DIAGNOSTICA MICROBIOLOGICA | |
| 2 | 1 Principi di microbiologia | Koneman's 7ed. cap. 1, 5, altro materiale su Studium |
| 3 | 2 Diagnostica delle malattie batteriche | Koneman's 7ed. cap. 2, 3, 4, 17, altro materiale su Studium |
| 4 | 3 Diagnostica delle malattie virali | Koneman's 7ed. cap. 2, 3, 4, 23 (1501-1571) |
| 5 | 4 Diagnostica delle malattie da funghi | Koneman's 7ed. cap. 2, 3, 4, 21 |
| 6 | 5 Il controllo di qualità microbiologico e l’igiene dell’ambiente lavoro |