Professional laboratory

Academic Year 2025/2026 - Teacher: Davide SPARTA'

Expected Learning Outcomes

Expected Learning Outcomes

Achievement of professional knowledge through theoretical and practical knowledge that enables, respectively, both the learning of quality control and assessment elements of the analytical phase and the performance of some basic laboratory medicine activities.

Acquisition of knowledge regarding the proper organization within an analysis laboratory, the main instruments in use, and their maintenance.

Knowledge of the methodologies and terminology used in emergency situations (POCT and TAT).

Teaching Method

Classroom lectures and practical laboratory exercises

Required Prerequisites

Basic knowledge of: General Chemistry - Biology - Physiology - Computer Science

Lecture Attendance

Mandatory for a minimum of 70% of the required credits.

Course Content

Knowledge of the analytical phase in laboratory medicine, with particular emphasis on the use of Quality Controls and their control charts.

Theoretical and practical exercises on laboratory procedures, with particular reference to microbiology.

Theoretical and practical exercises on liquid volume measurements (solution preparation and biological sample dilution techniques).

Reference Texts

M. Fiore, M. Ferrante, “The Research Laboratory: A Theoretical-Practical Manual” -- LSWR

Course Schedule

Topics Reference Texts

1 1. Theoretical Knowledge of the Main Analytical Techniques

2 2. Technical Validation

3 3. Culture, Enzyme Immunoassays, and Biochemical Tests in Microbiology

4 4. Theoretical-Practical Exercise on the Detection of a Pathogenic Bacterium in Foods of Animal Origin

5 5. Enumeration of Pathogenic Bacteria

6 6. Enzyme Immunoassays in Microbiology

7 7. Expression of Analytical Results in Microbiology

8 8. Laboratory Quality

9 9. Differences between “Accredia” Accredited and Non-Accredited Laboratories

10 10. Use of Control Charts

11 11. Internal Quality Control, External Quality Control, EQA, and Ring Test

12 12. Comparison of Technologies in the Analytical Phase (T.L.A. – Corelab – POCT)

13 13. The Laboratory in Emergency Care: POCT and TAT

14 14. Management of Control Charts and Possible Corrective Actions for the Analytical Process for "Out of Control" Values

15 15. Techniques in Immunohematology (Blood Transfusion Centers)

16 16. Expressions Used to Define the Concentration of a Solution (Percentage - Molarity - Normality)

17 17. Theoretical and Practical Exercises on Hematological Staining Techniques for the Morphological Study of Blood Cells

Detailed Course Content

Expected Learning Outcomes

Achievement of professional knowledge through theoretical and practical knowledge that enables, respectively, both the learning of control and evaluation elements of the pre-analytical and analytical phases and the performance of some basic laboratory medicine activities.

Teaching Method

Classroom lectures and practical laboratory exercises.

Prerequisites

Basic knowledge of: General Chemistry, Biology, Physiology, and Computer Science.

Attendance

Mandatory for a minimum of 70% of the required credits.

Course Content

Knowledge of the professional skills of the Biomedical Laboratory Technician, the professional code of ethics, and all the techniques and tools used to determine analytical results.

Reference Texts

M. Fiore, M. Ferrante “The Research Laboratory: A Theoretical-Practical Manual” -- LSWR

Course Schedule

Topics Reference Texts

1 1. Job Description of the Biomedical Laboratory Technician

2 2. Skills of the Biomedical Laboratory Technician

3 3. The Code of Ethics of the Biomedical Laboratory Technician

4 4. Career Opportunities

5 5. Notes on the Collection of Biological Samples

6 6. Transport and Storage of Biological Samples

7 7. Appropriateness of Requests

8 8. Notes on the Basic Instrumentation Commonly Used in a Laboratory Analysis

9 9. Theoretical-Practical Exercise on the Use of Basic Laboratory Instrumentation

10 10. Quality in the Laboratory

11 11. External Quality Control, EQA, and Ring Test

12 12. Main Analytical Techniques in Microbiology

13. Culture, immunoenzymatic, and biochemical tests in microbiology

14. Expression of analytical results in microbiology

15. Theoretical and practical exercises on the detection of pathogenic bacteria in foods of animal origin

Course Planning

 SubjectsText References
11. Conoscenze teoriche delle principali tecniche analitiche
22. Validazione tecnica
33. Esami colturali, immunoenzimatici e biochimici in microbiologia
44. Esercitazione teorico pratico sulla ricerca di un batterio patogeno negli alimenti di origine animale
55. Enumerazioni di batteri patogeni
66. Analisi immunoenzimatiche in microbiologia
77. Espressione del risultato analitico in microbiologia
88. Qualità in laboratorio
99. Differenza tra Laboratorio Accreditato “Accredia” e non accreditato
1010. Uso delle carte di controllo
1111. Controllo di qualità interno, Controllo di qualità Esterno, VEQ e Ring Test
1212. Tecnologie a confronto nella fase analitica (T.L.A. – Corelab – POCT)
1313. Il laboratorio nelle urgenze: POCT e TAT
1414. Gestione delle carte di controllo e possibili - Azioni correttive del processo analitico per i valori “fuori controllo''
1515. Tecniche in immunoematologia (centri trasfusionali
1616. Espressioni in uso per definire la concentrazione di una soluzione (percentuale - molarità - normailtà)
1717. Esercitazioni teorico pratico sulle tecniche di colorazioni ematologiche per lo studio morfologico degli elementi corpuscolati del sangue
VERSIONE IN ITALIANO