Professional laboratory
Academic Year 2025/2026 - Teacher: Davide SPARTA'Expected Learning Outcomes
Expected Learning Outcomes
Achievement of professional knowledge through theoretical and practical knowledge that enables, respectively, both the learning of quality control and assessment elements of the analytical phase and the performance of some basic laboratory medicine activities.
Acquisition of knowledge regarding the proper organization within an analysis laboratory, the main instruments in use, and their maintenance.
Knowledge of the methodologies and terminology used in emergency situations (POCT and TAT).
Teaching Method
Classroom lectures and practical laboratory exercises
Required Prerequisites
Basic knowledge of: General Chemistry - Biology - Physiology - Computer Science
Lecture Attendance
Mandatory for a minimum of 70% of the required credits.
Course Content
Knowledge of the analytical phase in laboratory medicine, with particular emphasis on the use of Quality Controls and their control charts.
Theoretical and practical exercises on laboratory procedures, with particular reference to microbiology.
Theoretical and practical exercises on liquid volume measurements (solution preparation and biological sample dilution techniques).
Reference Texts
M. Fiore, M. Ferrante, “The Research Laboratory: A Theoretical-Practical Manual” -- LSWR
Course Schedule
Topics Reference Texts
1 1. Theoretical Knowledge of the Main Analytical Techniques
2 2. Technical Validation
3 3. Culture, Enzyme Immunoassays, and Biochemical Tests in Microbiology
4 4. Theoretical-Practical Exercise on the Detection of a Pathogenic Bacterium in Foods of Animal Origin
5 5. Enumeration of Pathogenic Bacteria
6 6. Enzyme Immunoassays in Microbiology
7 7. Expression of Analytical Results in Microbiology
8 8. Laboratory Quality
9 9. Differences between “Accredia” Accredited and Non-Accredited Laboratories
10 10. Use of Control Charts
11 11. Internal Quality Control, External Quality Control, EQA, and Ring Test
12 12. Comparison of Technologies in the Analytical Phase (T.L.A. – Corelab – POCT)
13 13. The Laboratory in Emergency Care: POCT and TAT
14 14. Management of Control Charts and Possible Corrective Actions for the Analytical Process for "Out of Control" Values
15 15. Techniques in Immunohematology (Blood Transfusion Centers)
16 16. Expressions Used to Define the Concentration of a Solution (Percentage - Molarity - Normality)
17 17. Theoretical and Practical Exercises on Hematological Staining Techniques for the Morphological Study of Blood Cells
Detailed Course Content
Expected Learning Outcomes
Achievement of professional knowledge through theoretical and practical knowledge that enables, respectively, both the learning of control and evaluation elements of the pre-analytical and analytical phases and the performance of some basic laboratory medicine activities.
Teaching Method
Classroom lectures and practical laboratory exercises.
Prerequisites
Basic knowledge of: General Chemistry, Biology, Physiology, and Computer Science.
Attendance
Mandatory for a minimum of 70% of the required credits.
Course Content
Knowledge of the professional skills of the Biomedical Laboratory Technician, the professional code of ethics, and all the techniques and tools used to determine analytical results.
Reference Texts
M. Fiore, M. Ferrante “The Research Laboratory: A Theoretical-Practical Manual” -- LSWR
Course Schedule
Topics Reference Texts
1 1. Job Description of the Biomedical Laboratory Technician
2 2. Skills of the Biomedical Laboratory Technician
3 3. The Code of Ethics of the Biomedical Laboratory Technician
4 4. Career Opportunities
5 5. Notes on the Collection of Biological Samples
6 6. Transport and Storage of Biological Samples
7 7. Appropriateness of Requests
8 8. Notes on the Basic Instrumentation Commonly Used in a Laboratory Analysis
9 9. Theoretical-Practical Exercise on the Use of Basic Laboratory Instrumentation
10 10. Quality in the Laboratory
11 11. External Quality Control, EQA, and Ring Test
12 12. Main Analytical Techniques in Microbiology
13. Culture, immunoenzymatic, and biochemical tests in microbiology
14. Expression of analytical results in microbiology
15. Theoretical and practical exercises on the detection of pathogenic bacteria in foods of animal origin
Course Planning
| Subjects | Text References | |
|---|---|---|
| 1 | 1. Conoscenze teoriche delle principali tecniche analitiche | |
| 2 | 2. Validazione tecnica | |
| 3 | 3. Esami colturali, immunoenzimatici e biochimici in microbiologia | |
| 4 | 4. Esercitazione teorico pratico sulla ricerca di un batterio patogeno negli alimenti di origine animale | |
| 5 | 5. Enumerazioni di batteri patogeni | |
| 6 | 6. Analisi immunoenzimatiche in microbiologia | |
| 7 | 7. Espressione del risultato analitico in microbiologia | |
| 8 | 8. Qualità in laboratorio | |
| 9 | 9. Differenza tra Laboratorio Accreditato “Accredia” e non accreditato | |
| 10 | 10. Uso delle carte di controllo | |
| 11 | 11. Controllo di qualità interno, Controllo di qualità Esterno, VEQ e Ring Test | |
| 12 | 12. Tecnologie a confronto nella fase analitica (T.L.A. – Corelab – POCT) | |
| 13 | 13. Il laboratorio nelle urgenze: POCT e TAT | |
| 14 | 14. Gestione delle carte di controllo e possibili - Azioni correttive del processo analitico per i valori “fuori controllo'' | |
| 15 | 15. Tecniche in immunoematologia (centri trasfusionali | |
| 16 | 16. Espressioni in uso per definire la concentrazione di una soluzione (percentuale - molarità - normailtà) | |
| 17 | 17. Esercitazioni teorico pratico sulle tecniche di colorazioni ematologiche per lo studio morfologico degli elementi corpuscolati del sangue |