METODICHE DIAGNOSTICHE DI MICROBIOLOGIA CLINICA E PARASSITOLOGIA

Learning Objectives

• TECNICHE IN DIAGNOSTICA MICROBIOLOGICA

  TECHNIQUES FOR DIAGNOSTIC MICROBIOLOGY

  Knowledge of applications foundation for microbiological sciences in the various sectors of health interest

  Knowledge of the main microbial identification techniques and acquisition of the skills to apply the same in health care

  Knowledge of the "Guidelines for sterilization on the activity as a collective protection against biological agents for the operator in health facilities (Legislative Decree no. 81/2008 and subsequent amendments)"

Course Structure

• TECNICHE IN DIAGNOSTICA MICROBIOLOGICA

  TECHNIQUES FOR DIAGNOSTIC MICROBIOLOGY

   

  Lectures

  Should teaching be carried out in mixed mode or remotely, it may be necessary to introduce changes with respect to previous statements, in line with the programme planned and outlined in the syllabus.

  Learning assessment may also be carried out on line, should the conditions require it.”

Detailed Course Content

• TECNICHE IN DIAGNOSTICA MICROBIOLOGICA

  TECHNIQUES FOR DIAGNOSTIC MICROBIOLOGY Principles of Microbiology

  Microbes and metazoa
  Principles of microbial nutrition and metabolism
  Methods of microbial taxonomy and nomenclatureMetodi biochimici

  Molecular methods
  Other methods (not molecular) New approaches to taxonomy Omic Sciences

  Diagnostics of bacterial diseases

  The methods of sampling
  Transport media for bacteriology

  Microscopic examination Staining techniques

  Direct diagnostics Cultivation methods

  Culture media
  Aerobic and anaerobic growth
  Microorganisms that require special nutrients and/or cells for growth Biochemical tests for identification

  Manual methods

  Automated methods Blood Culture

  Culture independent and molecular techniques Immunofluorescence

  Enzyme immuno assay Other methods Molecular methods

  Hybridization Probes

  Amplification techniques
  Typing systems
  Other culture independent techniques

  Indirect diagnostic: Serological methods

  Immunofluorescence Enzyme immuno assay Other methods

   

  Susceptibility testing and bioassay
  In vitro antibiotic susceptibility testing

  Dilution methods Diffusion methods Automated methods

  Bioassays
  Pyrogenes and bacterial endotoxins Pyrogenes assay
  Assay for bacterial endotoxins (LAL test) Biological Assay of antibiotics
  Assay of interferons
  Assessment of the activity of disinfectants

  Diagnostic of viral diseases

  The methods of sampling Cultivation method

  The components required for cell culture media. Primary and Secondary cell cultures Semi-continuous diploid cells (Cell strains) Continuous cell lines

  Culture independent and molecular techniques Enzyme Immuno assay

  Immunofluorescence Molecular methods

  Hybridization
  Probes
  Amplification techniques Typing systems

  Other culture independent techniques Serological methods

  Immunofluorescence Enzyme immuno assay Other methods

  Antiviral chemotherapy
  The assay of substances with potential antiviral activity

  Diagnostic of fungal diseases

  The methods of sampling Microscopy and culture

  Staining techniques
  Mycological culture media
  Biochimical test for fungal identification

  Manual

  Automated
  Culture independent and molecular techniques

  Immunofluorescence Enzyme immuno assay

   

  Other methods

  Molecular methods Hybridization

  Probes
  Amplification techniques
  Typing systems
  Other culture independent techniques

  Serological methods Immunoflurescence

  Enzyme iImmuno assay

  Other technique Antimycotic susceptibility besting

  Dilution methods Yeast Molds

  Other technique

  The microbiological quality control and hygiene environment work [Notes on guidelines for the activity of sterilization as a collective protection against biological agents for the operator in health facilities (D.Lgs. 81/2008 e s.m.i.)]

  The sterilization process and its enterprise management People involved
  Structural and technological requirements.

  Large / medium-sized hospitals

  Small hospitals Operating protocols

  Harvesting
  Decontamination
  Washing (manual, ultrasaund, automated)
  Personal protective equipment [gloves, protective clothing, respiratory protection devices, for face protection (face shield or equivalent)]

  Rinsing, drying, inspection and maintenance
  Medical paper, envelopes and rolls in paper-laminated polymeric film, a polymeric material composition of various types usable in sheets, polyolefin and similar materials suitable for use in rolls or tubular, reusable Material: container
  Sterilization (steam sterilization, with ethylene oxide, with other physical methods, gas plasma of hydrogen peroxide, peracetic acid solutions
  Controls on environments and the process steps

  Food Microbiology - HACCP System

  HACCP system
  Description of the HACCP system

  Preliminary phases of the application system Main features of HACCP system

  Principles 1 to 7 Food microbiology

   

  Meaning of microorganisms in food

  Unfermented foods

  Meat, poultry, eggs, seafood, vegetables and fruit Fermented foods

  Fermented Vegetables, bread, cheese, fermented milk, fermented meat products

Textbook Information

• TECNICHE IN DIAGNOSTICA MICROBIOLOGICA

  TECHNIQUES FOR DIAGNOSTIC MICROBIOLOGY

  Koneman's - Color Atlas and textbook of Diagnostic Microbiology 7ed. Wolters Kluver - International Edition ISBN-13: 978-1-4511-8935-3

  Other informations and papers will be available in Studium