SCIENZE DI MEDICINA DI LABORATORIO

Learning Objectives

• Clinical Biochemistry

  The course is designed to provide students with a good foundation of knowledge in all aspects of Clinical Biochemistry. Introducing the fundamentals of instrumentation and methodology in the clinical chemistry and biochemistry laboratory medicine, lectures will cover urinalysis testing procedures and associated disease entities, as well as analysis of other body fluids. Clinical testing using automated and manual methods, measurement of pancreatic function and intestinal absorption, renal and liver function, enzymes, electrolytes, blood gases, lipids, toxicology, urinalysis, endocrinology, neurological, dysmetabolic, inflammatory and cardiovascular diseases.

• Clinical Pathology

  The course develops skills in the Clinical Pathology aiming to teach the student to apply, evaluate and interpret the laboratory tests used to study of the main diseases of the blood, alterations of the coagulative-fibrinolytic system, changes in liver and kidney function, some endocrinopathies, dyslipidemia, myocardial infarction, autoimmune diseases and cancer.

• Institutions of Anatomic Pathology

  Knowledge of the basic of human pathology, in particular cellular, morphological, immunophenotypical and molecular alterations of inflammatory , immune and neoplastic diseases. Organization of the laboratory of pathology, in particular methods of histo-cytopathology, immunofluorescence, immunohistochemistry and molecular biology

Course Structure

• Clinical Biochemistry

  frontal teaching, elaboration and individual study

• Clinical Pathology

  The lessons are held in the classroom with the help of PDF presentations made available to students on the Studium portal. The presentations do not replace the reference texts, but serve as educational support for the program carried out. Should teaching be carried out in mixed mode or remotely, it may be necessary to introduce changes with respect to previous statements, in line with the programme planned and outlined in the syllabus.

  Learning assessment may also be carried out on line, should the conditions require it.
  

• Institutions of Anatomic Pathology

  Frontal teaching.

  Should teaching be carried out in mixed mode or remotely, it may be necessary to introduce changes with respect to previous statements, in line with the programme planned and outlined in the syllabus.

Detailed Course Content

• Clinical Biochemistry

  1) The use of laboratory data in clinical practice
  2) Analysis of Urine
  3) Biochemistry of nutrition
  4) Liver function and Hepatitis tests

  5) Blood cell count

  6) Diabetes mellitus
  7) Hypoglycemia
  8) Free radicals in the physiology and in the pathology
  9) Metabolism of ethanol and the pathology of alcoholism

  10) Tumor markers
  11) Notes on antiaging medicine.

• Clinical Pathology

  Laboratory analysis: definition, typology

  Collection and conservation of biological samples

  Preanalytical, analytical and biological variability

  Complete blood count test

  Plasma proteins and electrophoresis

  Hemostasis test

  Blood groups and compatibility testing

  Urine analysis and functional kidney test

  The functional liver and viral hepatitis tests

  Diabetes: diagnosis and follow up test

  Autoimmune disease test

• Institutions of Anatomic Pathology

  Techniques and diagnostic methodology in surgical Pathology.

  Purpose of the autopsy diagnostic.

  Purposes, indications and limits of the intraoperative examination.

  Indications to the various types of biopsy.

  Request for histopathological investigation.

  Diagnostic cytology.

  Application of immunohistochemistry and molecular biology techniques in anatomical and pathological diagnostics.

  Cell damage.

  Cellular response to stress.

  Reversible and irreversible cell damage.

  Apoptosis.

  Cell response to ischemia.

  Molecular mechanisms of cell death.

  Coagulative necrosis, colliquative, caseous, gummy, hemorrhagic, fatty and fibrinoid.

  Proliferation disorders and cell differentiation: hyperplasia, metaplasia and neoplasia.

  The cell cycle.

  The neoplasm: benign vs malignant.

  Prenoplastic lesions: in situ dysplasia and neoplasm.

  Morphological aspects of invasiveness and metastasis.

  Histological degree of malignancy.

  Staging (TNM system).

  Nomenclature and classification of neoplasms.

  Molecular definition of neoplasms.

  Inflammation and pathology of the immune system.

  Acute and chronic inflammation.

  Primitive and secondary immunodeficiencies.

  Histological alterations of lymph nodes and primary and peripheral lymphatic tissues.

  Histological aspects of infectious complications of immunodeficiencies: bacterial, fungal, viral and protozoal infections.

  Neoplasms associated with immunodeficiencies.

  Organ-specific autoimmune diseases (S.Sjogren, S.Goodpasture, M.Graves) and systemic diseases (LES, rheumatoid arthritis, scleroderma).

  The rejection of transplants.

Textbook Information

• Clinical Biochemistry

  Medicina di Laboratorio, G. Federici (Autore), – Mc GrawHill Medicina di laboratorio.

  Medicina di Laboratorio. La diagnosi di malattia nel laboratorio clinico, M. Laposata (Autore) - Piccin

  Medicina di Laboratorio. Logica e patologia clinica, I. Antonozzi, E. Gulletta (Autori) - Piccin

  Medicina dell'Aging e dell'Antiaging. V. Calabrese et al. (Autori) - Edra

  Nutrigenomica e Epigenetica. V. Calabrese et al. (Autori) - Edra

• Clinical Pathology

  1. Federici G. Medicina di Laboratorio. Mc Graw-Hill Companies

  2. Sacchetti L., Cavalcanti P. et. al. Medicina di laboratorio e diagnostica genetica. Sorbona

  2. Laposata M. Medicina di Laboratorio. Piccin

  3. Antonozzi I. Medicina di Laboratorio. Piccin

  5. Balestrieri et al. Diagnostica Molecolare nella Medicina di Laboratorio. Piccin

• Institutions of Anatomic Pathology

  A.Scarpa, L.Ruco - Anatomia patologica. Le basi

  Gallo-D’Amati - Anatomia Patologica Sistematica.