METODOLOGIE DIAGNOSTICHE DI ANATOMIA PATOLOGICA

Academic Year 2020/2021 - 3° Year
Teaching Staff Credit Value: 7
Scientific field: MED/08 - Pathology
Taught classes: 49 hours
Term / Semester:
ENGLISH VERSION

Learning Objectives

  • Sistematic Anatomic Pathology

    Knowledge of the morphological and molecular features, nosological classification and differential diagnosis of the main diseases. Knowledge of early diagnosis, prognostic and predictive factors of neoplastic diseases.

  • Diagnostic Cytopathology

    At the end of the course the student will have to know the main types of diagnostic cytopathology exams, be able to set up and color the cytological preparations, know the basic principles of reactive and neoplastic cellular pathology; it must also be able to apply the general concepts of cytopathology to the main types of cytological samples.

  • Immunohistochemistry and Molecular Pathology

    Knowledge of the main immunohistochemical and biomolecular techniques.


Course Structure

  • Sistematic Anatomic Pathology

    Frontal teaching.

     

    Should teaching be carried out in mixed mode or remotely, it may be necessary to introduce changes with respect to previous statements, in line with the programme planned and outlined in the syllabus.

  • Diagnostic Cytopathology

    Biweekly.

    Should teaching be carried out in mixed mode or remotely, it may be necessary to introduce changes with respect to previous statements, in line with the programme planned and outlined in the syllabus.

  • Immunohistochemistry and Molecular Pathology

    Frontal teaching.

    Should teaching be carried out in mixed mode or remotely, it may be necessary to introduce changes with respect to previous statements, in line with the programme planned and outlined in the syllabus.


Detailed Course Content

  • Sistematic Anatomic Pathology

    Cellular lesions: hypertrophy, hyperplasia, metaplasia, dysplasia, carcinoma in situ, invasive carcinoma.

    Metastasis: cellular mechanisms and metastatic pathways (lymphatic, hematic, transperitoneal).

    Classification and pathological basis of malignant tumors: carcinomas, leukemia / lymphomas, sarcomas, neuroendocrine tumors, melanomas, brain tumors.

    Anatomopathological bases of the most common carcinomas: lung, breast, thyroid, gastric, colo-rectal, hepatic, renal, prostatic, bladder carcinoma.

    Anatomopathological diagnostics: cytological examinations (exfoliative cytology, faaspirative cytology); biopsy (endoscopy, needle biopsy, incisional biopsy and excisional biopsy); extemporaneous examination at the freezer.

    Staging of malignant neoplasms (TNM).

  • Diagnostic Cytopathology

    Diagnostic cytopathology: advantages and limitations

    The different cytologic samples: needle and aspiration cytology

    Technique of cytological preparations: smear, thin, cell-block, cytocentrifugation

    Fixation and histochemical staining of cytological samples

    Techniques of immunocytochemistry, flow cytometry and FISH in diagnostic cytology

    Evaluation of cytological samples: criteria of adequacy

    Evaluation of morphological characteristics of the cells: normal cells, hyperplastic, atypical cells

    False negatives and false positives

    Diagnostic Cytopathology of thyroid

    Diagnostic Cytopathology of the lung

    Diagnostic cytopathology of the female genital tract: PAP tests and breast cytology

    Diagnostic Cytopathology of the urinary tract

    Diagnostic Cytopathology of the effusions

  • Immunohistochemistry and Molecular Pathology

    Enzymatic histochemistry (special stains: eg Alcian-PAS, Giemsa, Rosso Congo, etc.)

    immunohistochemistry: unmasking, detection systems, antigen-antibody reaction, primary and secondary antibodies

    Main molecular biology techniques (FISH, CISH)


Textbook Information

  • Sistematic Anatomic Pathology

    Ruco-Scarpa Anatomia Patologica – Le Basi UTET

  • Diagnostic Cytopathology

    Anatomia Patologica- Le basi- Luigi Ruco, Aldo Scarpa. Edizioni UTET

  • Immunohistochemistry and Molecular Pathology

    Ruco Scarpa- Anatomia Patologica- Le basi, UTET